Name: Advancement: S. Seiler - Automating the culture of stress-reduced cerebral organoids for large-scale experiments with IoT-connected microfluidics and real-time feedback
Start: 2023-05-08T13:15:00-07:00
End: 2023-05-08
Location: Biomedical Science, Rm 300

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Biomedical Science, Rm 300

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Description: Organ-on-a-chip systems combine microfluidics, cell biology, and tissue engineering to culture 3D organ-specific in vitro models that recapitulate the biology and physiology of their \textit{in vivo} counterparts. The cerebral cortex organoid have facilitated in vitro research on many aspects of neurology, however, the current model presents drawbacks that limit the scale of studies, control of environmental factors, and the metabolic health of cultures. The proposal here is to develop a multiplex platform that automates the culture of individual organoids in isolated microenvironments at user-defined media flow rates and custom patterning protocols. The platform will be enhanced with novel microfluidic interfaces and sensors to achieve greater throughput and self-correcting feedback algorithms for quality control the inputs of images and analytes measured from the conditioned media.

Finally, optimization experiments will be run on the platform in the form of gradient sets to study the feeding rate, media composition, and factor concentration that are all aimed to increase homeostasis in the organoid microenvironment similarity to in vivo environments. The capstone experiment of this proposal is a study of cerebral organoids expanded and maintained in feed-optimized, stress-reduced automated culture to compare differences vs. conventional organoid protocols in cell sub-type populations, growth morphology, and RNA expression. Data from the initial study from the prototype platform presented RNA sequencing (RNA-seq) results of automated cerebral organoid cultures that showed benefits in reducing glycolytic and endoplasmic reticulum stress compared to conventional in vitro cell cultures.

Event Host: Spencer Seiler, Ph.D. Student, Biomolecular Engineering & Bioinformatics

Advisor: David Haussler and Mircea Teodorescu

Join us in person or on Zoom: https://ucsc.zoom.us/j/3614672960?pwd=M0tlRW81aUZYNk9sdk4xSjVOUjFEZz09

Advancement: S. Seiler - Automating the culture of stress-reduced cerebral organoids for large-scale experiments with IoT-connected microfluidics and real-time feedback

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